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验证数据展示

  • WB analysis using 26430-1-AP

    WB analysis using 26430-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26430-1-AP (BOK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 26430-1-AP

    FC experiment of MCF-7 using 26430-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human BOK (26430-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

BOK Polyclonal antibody

BOK Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 26430-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

BCL2L9, hBOK, BOKL, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26430-1-AP targets BOK in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 BOK fusion protein Ag24070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-related ovarian killer
别名 BCL2L9, hBOK, BOKL, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC006203
基因名称 BOK
Gene ID (NCBI) 666
RRIDAB_3085869
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BOK belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family.

实验方案

Product Specific Protocols
WB protocol for BOK antibody 26430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols