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BORIS Polyclonal antibody

BORIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11074-2-AP

Print datasheet

Synonyms

CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27

验证数据展示

  • WB analysis of PC-3 using 11074-2-AP

    WB analysis of PC-3 using 11074-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 11074-2-AP

    IF Staining of SH-SY5Y using 11074-2-AP

    Immunofluorescent analysis of SH-SY5Y7 cells, using CTCFL antibody 11074-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human cervical cancer using 11074-2-AP

    IHC staining of human cervical cancer using 11074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCFL/BORIS antibody included
  • IHC staining of human cervical cancer using 11074-2-AP

    IHC staining of human cervical cancer using 11074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCFL/BORIS antibody included

经过测试的应用

Positive WB detected inPC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11074-2-AP targets BORIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BORIS fusion protein Ag1547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCCTC-binding factor (zinc finger protein)-like
Synonyms CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27
Calculated Molecular Weight 82 kDa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC130486
Gene Symbol BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_614983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NI51
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. Present polyclonal anti-CFCFL antibody(11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detect a 75-kDa band and a 55-kDa band.

实验方案

Product Specific Protocols
WB protocol for BORIS antibody 11074-2-APDownload protocol
IHC protocol for BORIS antibody 11074-2-APDownload protocol
IF protocol for BORIS antibody 11074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols