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验证数据展示

  • WB analysis of PC-3 using 11074-2-AP

    WB analysis of PC-3 using 11074-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 11074-2-AP

    IHC staining of human cervical cancer using 11074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCFL/BORIS antibody included
  • IHC staining of human cervical cancer using 11074-2-AP

    IHC staining of human cervical cancer using 11074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTCFL/BORIS antibody included
  • IF Staining of HeLa using 11074-2-AP

    IF Staining of HeLa using 11074-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BORIS antibody (11074-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BORIS Polyclonal antibody

BORIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11074-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11074-2-AP targets BORIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BORIS fusion protein Ag1547 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCCTC-binding factor (zinc finger protein)-like
别名 CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27
计算分子量 82 kDa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC130486
基因名称 BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_614983
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NI51
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. Present polyclonal anti-CFCFL antibody(11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detect a 75-kDa band and a 55-kDa band.

实验方案

Product Specific Protocols
WB protocol for BORIS antibody 11074-2-APDownload protocol
IHC protocol for BORIS antibody 11074-2-APDownload protocol
IF protocol for BORIS antibody 11074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols