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验证数据展示

  • WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

BORIS Monoclonal antibody, PBS Only

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60079-1-PBS

产品说明书

CloneNo. 2B12E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.

产品信息

60079-1-PBS targets BORIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 BORIS fusion protein Ag1003 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CCCTC-binding factor (zinc finger protein)-like
别名 BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T
计算分子量 82 kDa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC130486
基因名称 BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_1211604
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NI51
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.