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BORIS Monoclonal antibody, PBS Only

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12E2

Cat no : 60079-1-PBS

Print datasheet

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

验证数据展示

  • WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.

产品信息

60079-1-PBS targets BORIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen BORIS fusion protein Ag1003 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CCCTC-binding factor (zinc finger protein)-like
Synonyms BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T
Calculated Molecular Weight 82 kDa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC130486
Gene Symbol BORIS/CTCFL
Gene ID (NCBI) 140690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NI51
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.