验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy BPTF Ready-To-Use IHC Kit. BPTF primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28016-1-AP targets BPTF in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | BPTF fusion protein Ag27761 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bromodomain PHD finger transcription factor |
| 别名 | BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301 |
| 计算分子量 | 338 kDa |
| GenBank蛋白编号 | NM_004459 |
| 基因名称 | BPTF |
| Gene ID (NCBI) | 2186 |
| RRID | AB_2918139 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12830 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BPTF antibody 28016-1-AP | Download protocol |
| IF protocol for BPTF antibody 28016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
