• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BPTF Polyclonal antibody

BPTF Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 28016-1-AP

Print datasheet

Synonyms

BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301

验证数据展示

  • IHC staining of human breast cancer using 28016-1-AP

    IHC staining of human breast cancer using 28016-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BPTF antibody included
  • IF Staining of HepG2 using 28016-1-AP

    IF Staining of HepG2 using 28016-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BPTF antibody (28016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BPTF Ready-To-Use IHC Kit. BPTF primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28016-1-AP targets BPTF in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, ChIP
Tested Reactivity Human
Cited Reactivityhuman
Immunogen BPTF fusion protein Ag27761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain PHD finger transcription factor
Synonyms BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301
Calculated Molecular Weight 338 kDa
GenBank Accession NumberNM_004459
Gene Symbol BPTF
Gene ID (NCBI) 2186
RRIDAB_2918139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)

实验方案

Product Specific Protocols
IHC protocol for BPTF antibody 28016-1-APDownload protocol
IF protocol for BPTF antibody 28016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,ChIP

Biomolecules

Lumbrokinase Extracted from Earthworms Synergizes with Bevacizumab and Chemotherapeutics in Treating Non-Small Cell Lung Cancer by Targeted Inactivation of BPTF/VEGF and NF-κB/COX-2 Signaling

Authors - Chunyu Hua
View Article