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BRAP Polyclonal antibody

BRAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26004-1-AP

Print datasheet

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

验证数据展示

  • WB analysis of MCF-7 using 26004-1-AP

    WB analysis of MCF-7 using 26004-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26004-1-AP (BRAP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26004-1-AP

    IHC staining of human kidney using 26004-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26004-1-AP

    IHC staining of human kidney using 26004-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26004-1-AP is BRAP Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen BRAP fusion protein Ag23279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BRCA1 associated protein
Synonyms BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52
Calculated Molecular Weight 592 aa, 67 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
RRIDAB_2880330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BRAP antibody 26004-1-APDownload protocol
IHC protocol for BRAP antibody 26004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols