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BRAP Polyclonal antibody

BRAP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27431-1-AP

Print datasheet

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

验证数据展示

  • WB analysis of MCF-7 using 27431-1-AP

    WB analysis of MCF-7 using 27431-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27431-1-AP

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27431-1-AP

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of MCF-7 using 27431-1-AP

    IP experiment of MCF-7 using 27431-1-AP

    IP result of anti-BRAP (IP:27431-1-AP, 4ug; Detection:27431-1-AP 1:1000) with MCF-7 cells lysate 3200 ug.

  • IHC staining of mouse testis using 27431-1-AP

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27431-1-AP

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27431-1-AP targets BRAP in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen BRAP fusion protein Ag26728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BRCA1 associated protein
Synonyms BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52
Calculated Molecular Weight 592 aa, 67 kDa
Observed Molecular Weight 62-70 kDa, 48 kDa
GenBank Accession NumberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
RRIDAB_2880869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BRAP antibody 27431-1-APDownload protocol
IHC protocol for BRAP antibody 27431-1-APDownload protocol
IP protocol for BRAP antibody 27431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols