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  • Featured Product
  • KD/KO Validated

BRD4 Polyclonal antibody

BRD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28486-1-AP

Print datasheet

Synonyms

Protein HUNK1, MCAP, HUNKI, Bromodomain-containing protein 4, bromodomain containing 4

验证数据展示

  • WB analysis using 28486-1-AP

    WB analysis using 28486-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 28486-1-AP

    IP experiment of A549 using 28486-1-AP

    IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug.

  • WB analysis using 28486-1-AP

    WB analysis using 28486-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28486-1-AP

    IHC staining of human lung cancer using 28486-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRD4 antibody included
  • IHC staining of human lung cancer using 28486-1-AP

    IHC staining of human lung cancer using 28486-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRD4 antibody included
  • IF Staining of HepG2 using 28486-1-AP

    IF Staining of HepG2 using 28486-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, MCF-7 cells, Ramos cells, Jurkat cells, MDA-MB-231 cells, T-47D cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28486-1-AP targets BRD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain containing 4
Synonyms Protein HUNK1, MCAP, HUNKI, Bromodomain-containing protein 4, bromodomain containing 4
Calculated Molecular Weight 1362 aa, 152 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC035266
Gene Symbol BRD4
Gene ID (NCBI) 23476
RRIDAB_2918170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members.

实验方案

Product Specific Protocols
WB protocol for BRD4 antibody 28486-1-APDownload protocol
IHC protocol for BRD4 antibody 28486-1-APDownload protocol
IF protocol for BRD4 antibody 28486-1-APDownload protocol
IP protocol for BRD4 antibody 28486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

Authors - Yi-Chang Wang
View Article
human,mouseWB,CoIP

Biochem Pharmacol

BRD4 promotes hepatic stellate cells activation and hepatic fibrosis via mediating P300/H3K27ac/PLK1 axis

Authors - Miao Cheng
  • KD Validated
View Article
humanWB

Cell Rep

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination

Authors - Yi-Chang Wang
View Article
humanWB

J Transl Med

Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation

Authors - Qinghui Kan
View Article