验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
ChIP | See 1 publications below |
产品信息
67374-2-Ig targets BRD4 in WB, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, ChIP |
经测试反应性 | Human, Mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | BRD4 fusion protein Ag27545 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | bromodomain containing 4 |
别名 | BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1 |
计算分子量 | 1362 aa, 152 kDa |
观测分子量 | 200 kDa |
GenBank蛋白编号 | BC035266 |
基因名称 | BRD4 |
Gene ID (NCBI) | 23476 |
RRID | AB_2918488 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O60885 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for BRD4 antibody 67374-2-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Bioengineered Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p. | ||
Curr Med Sci ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation | ||
Nat Commun MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition | ||
Tissue Cell Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization
|