BRD4 Monoclonal antibody

BRD4 Monoclonal Antibody for WB, ELISA
Cat No. 67374-2-Ig

产品说明书

CloneNo. 2F7E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse and More (3)

应用

WB, ELISA and More (1)

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67374-2-Ig targets BRD4 in WB, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, ChIP
经测试反应性 Human, Mouse
文献引用反应性human, mouse, rat
免疫原 BRD4 fusion protein Ag27545 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bromodomain containing 4
别名 BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1
计算分子量 1362 aa, 152 kDa
观测分子量200 kDa
GenBank蛋白编号BC035266
基因名称 BRD4
Gene ID (NCBI) 23476
RRIDAB_2918488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60885
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BRD4 antibody 67374-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bioengineered

Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p.

Authors - Dan-Dan Zhu
humanWB,ChIP

Curr Med Sci

ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer

Authors - Yang-Kai Jiang
human

J Transl Med

Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation

Authors - Qinghui Kan
mouseWB

Nat Commun

MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition

Authors - Fan Tong
human,ratWB

Tissue Cell

Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization

Authors - Lei Zhu
  • KD Validated
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