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BRD8 Polyclonal antibody

BRD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10476-1-AP

Print datasheet

Synonyms

TrCP120, SMAP2, SMAP, p120, Bromodomain-containing protein 8

验证数据展示

  • WB analysis of HeLa using 10476-1-AP

    WB analysis of HeLa using 10476-1-AP

    WB result of BRD8 antibody (10476-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRD8 transfected HeLa cells.

  • WB analysis using 10476-1-AP

    WB analysis using 10476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 10476-1-AP

    IHC staining of human stomach using 10476-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10476-1-AP (BRD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10476-1-AP

    IF Staining of HeLa using 10476-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (10476-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, HuH-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10476-1-AP targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen BRD8 fusion protein Ag0790 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain containing 8
Synonyms TrCP120, SMAP2, SMAP, p120, Bromodomain-containing protein 8
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120-170 kDa
GenBank Accession NumberBC008039
Gene Symbol BRD8
Gene ID (NCBI) 10902
RRIDAB_2066027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)

实验方案

Product Specific Protocols
WB protocol for BRD8 antibody 10476-1-APDownload protocol
IHC protocol for BRD8 antibody 10476-1-APDownload protocol
IF protocol for BRD8 antibody 10476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Authors - Arnaud Obri
View Article
humanWB

Arch Biochem Biophys

BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5.

Authors - Zhaoxiang Yu
View Article