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  • Featured Product
  • KD/KO Validated

BRD8 Monoclonal antibody, PBS Only

BRD8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B6D6

Cat no : 60121-1-PBS

Print datasheet

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

验证数据展示

  • WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

    WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

    WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

    WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

    IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

产品信息

60121-1-PBS targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BRD8 fusion protein Ag0790 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name bromodomain containing 8
Synonyms BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008039
Gene Symbol BRD8
Gene ID (NCBI) 10902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0E9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].