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BRE Polyclonal antibody

BRE Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11702-1-AP

Print datasheet

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

验证数据展示

  • WB analysis using 11702-1-AP

    WB analysis using 11702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11702-1-AP (BRE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11702-1-AP

    IP experiment of HeLa using 11702-1-AP

    IP result of anti-BRE (IP:11702-1-AP, 4ug; Detection:11702-1-AP 1:1000) with HeLa cells lysate 2320 ug.

  • IHC staining of human gliomas using 11702-1-AP

    IHC staining of human gliomas using 11702-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11702-1-AP

    IHC staining of human gliomas using 11702-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

11702-1-AP targets BRE in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BRE fusion protein Ag2256 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain and reproductive organ-expressed (TNFRSF1A modulator)
Synonyms BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2
Calculated Molecular Weight 383 aa, 44 kDa
Observed Molecular Weight 40-44 kDa
GenBank Accession NumberBC001251
Gene Symbol BRE
Gene ID (NCBI) 9577
RRIDAB_2066049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

实验方案

Product Specific Protocols
WB protocol for BRE antibody 11702-1-APDownload protocol
IHC protocol for BRE antibody 11702-1-APDownload protocol
IP protocol for BRE antibody 11702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
View Article
humanWB

Genes Dev

NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.

Authors - Wang Bin B
View Article
humanWB

Int J Biol Sci

PPDPF Promotes the Progression and acts as an Antiapoptotic Protein in Non-Small Cell Lung Cancer.

Authors - Mu Yun
View Article