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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

BRF2 Polyclonal antibody, PBS Only

BRF2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12056-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12056-1-PBS targets BRF2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BRF2 fusion protein Ag2686 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
别名
计算分子量 47 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC010648
基因名称 BRF2
Gene ID (NCBI) 55290
RRIDAB_2218683
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAW0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.