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BRF2 Polyclonal antibody, PBS Only

BRF2 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12056-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

产品信息

12056-1-PBS targets BRF2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BRF2 fusion protein Ag2686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Synonyms
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC010648
Gene Symbol BRF2
Gene ID (NCBI) 55290
RRIDAB_2218683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAW0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.