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BRIP1(C-terminal) Polyclonal antibody

BRIP1(C-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20983-1-AP

Print datasheet

Synonyms

BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF

验证数据展示

  • WB analysis of HeLa using 20983-1-AP

    WB analysis of HeLa using 20983-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20983-1-AP (BRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20983-1-AP targets BRIP1(C-terminal) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BRCA1 interacting protein C-terminal helicase 1
Synonyms BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberNM_032043
Gene Symbol BACH1/BRIP1
Gene ID (NCBI) 83990
RRIDAB_2878782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRIP1, also named as BACH1 and FANCJ, belongs to the DEAD box helicase family and DEAH subfamily. It is a DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. It is involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Defects in BRIP1 are a cause of susceptibility to breast cancer (BC). Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ). The antibody is specific to BRIP1.

实验方案

Product Specific Protocols
WB protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols