• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

BRIP1(N-terminal) Polyclonal antibody

BRIP1(N-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24436-1-AP

Print datasheet

Synonyms

BACH1, BRIP1, BRIP1(N-terminal), FANCJ, OF

验证数据展示

  • WB analysis using 24436-1-AP

    WB analysis using 24436-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24436-1-AP (BRIP1(N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24436-1-AP

    WB analysis of HeLa using 24436-1-AP

    WB result of BRIP1(N-terminal) antibody (24436-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRIP1(N-terminal) transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24436-1-AP targets BRIP1(N-terminal) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BRIP1(N-terminal) fusion protein Ag19907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BRCA1 interacting protein C-terminal helicase 1
Synonyms BACH1, BRIP1, BRIP1(N-terminal), FANCJ, OF
Calculated Molecular Weight 1249 aa, 141 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC101472
Gene Symbol BACH1/BRIP1
Gene ID (NCBI) 83990
RRIDAB_2879548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

实验方案

Product Specific Protocols
WB protocol for BRIP1(N-terminal) antibody 24436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Authors - Jiao Zhao
View Article