验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue |
| Positive IHC detected in | human prostate cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 4 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | MCPH/BRIT1 fusion protein Ag2567 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microcephalin 1 |
| 别名 | BRIT1, MCPH1, MCT, Microcephalin, microcephalin 1 |
| 计算分子量 | 93 kDa |
| 观测分子量 | 66 kDa, 100 kDa |
| GenBank蛋白编号 | BC030702 |
| 基因名称 | MCPH1 |
| Gene ID (NCBI) | 79648 |
| RRID | AB_2142989 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NEM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| IHC protocol for MCPH/BRIT1 antibody 11962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.
| ||
Stem Cell Res Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.
| ||
FEBS J Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.
| ||
Genes (Basel) MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.
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