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  • Featured Product
  • KD/KO Validated

BRMS1 Polyclonal antibody

BRMS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16096-1-AP

Print datasheet

Synonyms

BRMS1

验证数据展示

  • IHC staining of human brain using 16096-1-AP

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRMS1 antibody included
  • IHC staining of human brain using 16096-1-AP

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRMS1 antibody included
  • WB analysis of MCF-7 using 16096-1-AP

    WB analysis of MCF-7 using 16096-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of MCF-7 using 16096-1-AP

    IF Staining of MCF-7 using 16096-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16096-1-AP (BRMS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy BRMS1 Ready-To-Use IHC Kit. BRMS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16096-1-AP targets BRMS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human
Cited Reactivityhuman
Immunogen BRMS1 fusion protein Ag8939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast cancer metastasis suppressor 1
Synonyms BRMS1
Calculated Molecular Weight 246 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC009834
Gene Symbol BRMS1
Gene ID (NCBI) 25855
RRIDAB_2878217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCU9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. BRMS1 has since been reported to suppress metastasis in a variety of cancer types such as melanoma, ovarian carcinoma, and non-small lung cancer.

实验方案

Product Specific Protocols
WB protocol for BRMS1 antibody 16096-1-APDownload protocol
IHC protocol for BRMS1 antibody 16096-1-APDownload protocol
IF protocol for BRMS1 antibody 16096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Oncol Rep

BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.

Authors - Jianming Dou
  • KD Validated
View Article
humanWB

Oncol Res

miR-346 Promotes HCC Progression by Suppressing Breast Cancer Metastasis Suppressor 1 Expression

Authors - Zhixian Guo
View Article