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BRUNOL5 Polyclonal antibody

BRUNOL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13557-1-AP

Print datasheet

Synonyms

CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5

验证数据展示

  • WB analysis using 13557-1-AP

    WB analysis using 13557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13557-1-AP (BRUNOL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13557-1-AP

    IHC staining of mouse brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 13557-1-AP

    IHC staining of rat brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-87 MG using 13557-1-AP

    IF Staining of U-87 MG using 13557-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using BRUNOL5 antibody (13557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, U-251 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13557-1-AP targets BRUNOL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BRUNOL5 fusion protein Ag4416 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bruno-like 5, RNA binding protein (Drosophila)
Synonyms CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5
Calculated Molecular Weight 485 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC028101
Gene Symbol BRUNOL5
Gene ID (NCBI) 60680
RRIDAB_1211624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6W0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRUNOL5, also named CELF5, is a 485 amino acid protein, which belongs to the CELF/BRUNOL family. BRUNOL5 is expressed in the brain and localizes in the nucleus. BRUNOL5 is an RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. BRUNOL5 specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. BRUNOL5 exists in two isoforms with MV 52 and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IHC protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IF protocol for BRUNOL5 antibody 13557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols