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BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19078-1-AP

Print datasheet

Synonyms

CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6

验证数据展示

  • WB analysis using 19078-1-AP

    WB analysis using 19078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19078-1-AP

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 19078-1-AP

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19078-1-AP

    IF Staining of HeLa using 19078-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19078-1-AP targets BRUNOL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen BRUNOL6 fusion protein Ag4970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bruno-like 6, RNA binding protein (Drosophila)
Synonyms CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6
Calculated Molecular Weight 481 aa, 50 kDa
Observed Molecular Weight 50 kDa, 39 kDa
GenBank Accession NumberBC030835
Gene Symbol BRUNOL6
Gene ID (NCBI) 60677
RRIDAB_2243662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96J87
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IHC protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IF protocol for BRUNOL6 antibody 19078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu
View Article