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验证数据展示

  • WB analysis using 19078-1-AP

    WB analysis using 19078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19078-1-AP

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 19078-1-AP

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19078-1-AP

    IF Staining of HeLa using 19078-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19078-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19078-1-AP targets BRUNOL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse
免疫原 BRUNOL6 fusion protein Ag4970 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bruno-like 6, RNA binding protein (Drosophila)
别名 CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6
计算分子量 481 aa, 50 kDa
观测分子量 50 kDa, 39 kDa
GenBank蛋白编号BC030835
基因名称 BRUNOL6
Gene ID (NCBI) 60677
RRIDAB_2243662
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J87
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IHC protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IF protocol for BRUNOL6 antibody 19078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu
View Article