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验证数据展示

  • WB analysis using 29293-1-AP

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 29293-1-AP

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29293-1-AP

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29293-1-AP

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

BRWD1 Polyclonal antibody

BRWD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29293-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Bromodomain and WD repeat-containing protein 1, C21orf107, N143, WD repeat containing protein 9, WD repeat-containing protein 9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29293-1-AP targets BRWD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30902

Product name: Recombinant human BRWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of BC064602

Sequence: MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain and WD repeat domain containing 1
别名 Bromodomain and WD repeat-containing protein 1, C21orf107, N143, WD repeat containing protein 9, WD repeat-containing protein 9
计算分子量 2320 aa, 263 kDa
观测分子量263 kDa
GenBank蛋白编号BC064602
基因名称 BRWD1
Gene ID (NCBI) 54014
RRIDAB_3086110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).

实验方案

Product Specific Protocols
IHC protocol for BRWD1 antibody 29293-1-APDownload protocol
WB protocol for BRWD1 antibody 29293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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