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BSAP,PAX5 Polyclonal antibody

BSAP,PAX5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26709-1-AP

Print datasheet

Synonyms

PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5



经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRamos cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26709-1-AP targets BSAP,PAX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen BSAP,PAX5 fusion protein Ag25147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paired box 5
Synonyms PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5
Calculated Molecular Weight 391 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC156927
Gene Symbol PAX5
Gene ID (NCBI) 5079
RRIDAB_2880608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02548
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

实验方案

Product Specific Protocols
WB protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IHC protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IP protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.

Authors - Runzhi Huang
humanIHC

J Cutan Pathol

Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.

Authors - Jennifer S Ko
mouseIHC

Mol Oncol

miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer

Authors - Shuai Wu
humanWB,IP

Free Radic Biol Med

Prohibitin 2 confers NADPH oxidase 1 -mediated cytosolic oxidative signaling to promote gastric cancer progression by ERK activation

Authors - Liang Xu
mouseWB

Cell Mol Immunol

Rack1 regulates B-cell development and function by binding to and stabilizing the transcription factor Pax5

Authors - Xueting Zhang