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CD157 Polyclonal antibody

CD157 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 16337-1-AP

Print datasheet

Synonyms

ADP ribosyl cyclase 2, Bone marrow stromal antigen 1, BST 1, BST1, cADPr hydrolase 2, CD157, Cyclic ADP ribose hydrolase 2

验证数据展示

  • WB analysis of A549 using 16337-1-AP

    WB analysis of A549 using 16337-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16337-1-AP (CD157 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 16337-1-AP

    IHC staining of human tonsillitis using 16337-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 16337-1-AP

    IHC staining of human tonsillitis using 16337-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16337-1-AP targets CD157 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen CD157 fusion protein Ag9491 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone marrow stromal cell antigen 1
Synonyms ADP ribosyl cyclase 2, Bone marrow stromal antigen 1, BST 1, BST1, cADPr hydrolase 2, CD157, Cyclic ADP ribose hydrolase 2
Calculated Molecular Weight 318 aa, 36 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC012095
Gene Symbol CD157
Gene ID (NCBI) 683
RRIDAB_2878244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. BST-1 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to the glycosylation (PMID: 8941363, 10491089).

实验方案

Product Specific Protocols
WB protocol for CD157 antibody 16337-1-APDownload protocol
IHC protocol for CD157 antibody 16337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

EMBO J

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

Authors - Quanjiang Zhang
View Article