• Featured Product
  • KD/KO验证

BST2 Polyclonal antibody

BST2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13560-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (2)

CD317, Tetherin, CD 317, BST-2, BST 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13560-1-AP targets BST2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human
文献引用反应性human
免疫原 BST2 fusion protein Ag4430 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone marrow stromal cell antigen 2
别名 CD317, Tetherin, CD 317, BST-2, BST 2
计算分子量 180 aa, 20 kDa
观测分子量 30-36 kDa, 50-70 kDa
GenBank蛋白编号BC033873
基因名称 BST2
Gene ID (NCBI) 684
RRIDAB_2067220
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks retrovirus release from the cell surface. Depleted upon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane protein, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 are 30-36 kDa (monomer form) and 50-70 kDa (dimer form) (PMID: 19196977; 21237475; 19737401; 37922253).

实验方案

Product Specific Protocols
WB protocol for BST2 antibody 13560-1-APDownload protocol
IHC protocol for BST2 antibody 13560-1-APDownload protocol
IF protocol for BST2 antibody 13560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

PMID: 24753610

Authors - Xuesen Zhao
humanWB, IF

Curr Biol

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.

Authors - Adrien Presle
  • KO Validated
humanWB

PLoS Pathog

PMID: 28910400

Authors - Yuxuan Fu
  • KO Validated
humanWB, IF

J Virol

Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.

Authors - Ran Yan
  • KD Validated
humanWB

J Virol

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Authors - Weidner Jessica M JM
Loading...