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验证数据展示

  • WB analysis using 82733-7-RR (same clone as 82733-7-PBS)

    WB analysis using 82733-7-RR (same clone as 82733-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82733-7-RR (BST2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82733-7-RR (same clone as 82733-7-PBS)

    WB analysis of HeLa using 82733-7-RR (same clone as 82733-7-PBS)

    WB result of BST2 antibody (82733-7-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

    IF Staining of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BST2 antibody (82733-7-RR, Clone: 7J24 ) at dilution of 1:125 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

    FC experiment of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BST2 Recombinant antibody (82733-7-RR, Clone:7J24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82733-7-RR

    Affinity and Kinetic Characterization of 82733-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82733-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

BST2 Recombinant antibody, PBS Only

BST2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82733-7-PBS

产品说明书

CloneNo. 7J24

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82733-7-PBS targets BST2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 BST2 fusion protein Ag4430 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone marrow stromal cell antigen 2
别名 CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2
计算分子量 180 aa, 20 kDa
观测分子量 30-36 kDa
GenBank蛋白编号BC033873
基因名称 BST2
Gene ID (NCBI) 684
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ10589
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).