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验证数据展示

  • WB analysis using 84190-7-RR

    WB analysis using 84190-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84190-7-RR (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84190-7-RR

    IHC staining of mouse liver using 84190-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84190-7-RR (BST2 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84190-7-RR

    IF Staining of HeLa using 84190-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 84190-7-RR

    IF Staining of HepG2 using 84190-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84190-7-RR

    Affinity and Kinetic Characterization of 84190-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84190-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

BST2 Recombinant antibody

BST2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84190-7-RR

产品说明书

CloneNo. 241513A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, THP-1 cells, human heart tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84190-7-RR targets BST2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone marrow stromal cell antigen 2
别名 CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2
计算分子量 180 aa, 20 kDa
观测分子量 30-36 kDa, 50-70 kDa
GenBank蛋白编号BC033873
基因名称 BST2
Gene ID (NCBI) 684
RRIDAB_3671743
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ10589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

实验方案

Product Specific Protocols
WB protocol for BST2 antibody 84190-7-RRDownload protocol
IHC protocol for BST2 antibody 84190-7-RRDownload protocol
IF protocol for BST2 antibody 84190-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols