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BST2 Recombinant antibody

BST2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241513A1

Cat no : 84190-7-RR

Print datasheet

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

验证数据展示

  • WB analysis using 84190-7-RR

    WB analysis using 84190-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84190-7-RR (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84190-7-RR

    IHC staining of mouse liver using 84190-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84190-7-RR (BST2 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84190-7-RR

    IF Staining of HeLa using 84190-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 84190-7-RR

    IF Staining of HepG2 using 84190-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84190-7-RR

    Affinity and Kinetic Characterization of 84190-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84190-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, THP-1 cells, human heart tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84190-7-RR targets BST2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name bone marrow stromal cell antigen 2
Synonyms CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa, 50-70 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ10589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

实验方案

Product Specific Protocols
WB protocol for BST2 antibody 84190-7-RRDownload protocol
IHC protocol for BST2 antibody 84190-7-RRDownload protocol
IF protocol for BST2 antibody 84190-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols