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BTBD1 Polyclonal antibody

BTBD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15859-1-AP

Print datasheet

Synonyms

BTB (POZ) domain containing 1, BTBD1, C15orf1, NS5ATP8

验证数据展示

  • WB analysis of HeLa using 15859-1-AP

    WB analysis of HeLa using 15859-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15859-1-AP (BTBD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15859-1-AP

    IHC staining of human testis using 15859-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15859-1-AP (BTBD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15859-1-AP

    IHC staining of human testis using 15859-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15859-1-AP (BTBD1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15859-1-AP targets BTBD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen BTBD1 fusion protein Ag8339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BTB (POZ) domain containing 1
Synonyms BTB (POZ) domain containing 1, BTBD1, C15orf1, NS5ATP8
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC028097
Gene Symbol BTBD1
Gene ID (NCBI) 53339
RRIDAB_2067248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0C5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTBD1 antibody 15859-1-APDownload protocol
IHC protocol for BTBD1 antibody 15859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors

Authors - Alice Meroni
View Article