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BTBD16 Polyclonal antibody

BTBD16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24733-1-AP

Print datasheet

Synonyms

BTB (POZ) domain containing 16, BTBD16, C10orf87

验证数据展示

  • IHC staining of human kidney using 24733-1-AP

    IHC staining of human kidney using 24733-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24733-1-AP (BTBD16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 24733-1-AP

    WB analysis of mouse testis using 24733-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24733-1-AP (BTBD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24733-1-AP targets BTBD16 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BTBD16 fusion protein Ag20672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BTB (POZ) domain containing 16
Synonyms BTB (POZ) domain containing 16, BTBD16, C10orf87
Calculated Molecular Weight 507 aa, 59 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC109262
Gene Symbol BTBD16
Gene ID (NCBI) 118663
RRIDAB_2879696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32M84
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTBD16 also named as BTB (POZ) domain containing 16 or C10orf87 is 506 amino acid protein, which one BTB domain. The molecular weight fo BTBD16 is 58kDa. The function of BTBD16 protein is still non-known.

实验方案

Product Specific Protocols
WB protocol for BTBD16 antibody 24733-1-APDownload protocol
IHC protocol for BTBD16 antibody 24733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols