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验证数据展示

  • WB analysis using 16330-1-AP

    WB analysis using 16330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16330-1-AP (BTD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16330-1-AP

    IHC staining of human liver using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16330-1-AP

    IHC staining of human liver using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16330-1-AP

    IHC staining of mouse kidney using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16330-1-AP

    IF Staining of HeLa using 16330-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTD antibody (16330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BTD Polyclonal antibody

BTD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16330-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.5.1.12, biotinidase, Biotinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16330-1-AP targets BTD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BTD fusion protein Ag9449 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 biotinidase
别名 EC:3.5.1.12, biotinidase, Biotinase
计算分子量 543 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC012099
基因名称 BTD
Gene ID (NCBI) 686
RRIDAB_2067512
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

实验方案

Product Specific Protocols
WB protocol for BTD antibody 16330-1-APDownload protocol
IHC protocol for BTD antibody 16330-1-APDownload protocol
IF protocol for BTD antibody 16330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols