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验证数据展示

  • WB analysis of human brain using 19753-1-AP

    WB analysis of human brain using 19753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 19753-1-AP

    IHC staining of human cervical cancer using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human cervical cancer using 19753-1-AP

    IHC staining of human cervical cancer using 19753-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 10x lens).

BTF3L3-Specific Polyclonal antibody

BTF3L3-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19753-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

BTF3L3, HUMBTFD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19753-1-AP targets BTF3L3-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic transcription factor 3, like 3
别名 BTF3L3, HUMBTFD
计算分子量 23 kDa
观测分子量 25-30 kDa
GenBank蛋白编号Q13892
基因名称 BTF3L3
Gene ID (NCBI) 132556
RRIDAB_10637858
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTF3L3 belongs to the NAC-beta family. It is a general transcription factor. It is required for the initiation of transcription. The antibody has no cross reaction to BTF3.

实验方案

Product Specific Protocols
WB protocol for BTF3L3-Specific antibody 19753-1-APDownload protocol
IHC protocol for BTF3L3-Specific antibody 19753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols