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验证数据展示

  • WB analysis using 20514-1-AP

    WB analysis using 20514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20514-1-AP (BTG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 40x lens).

BTG4 Polyclonal antibody

BTG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20514-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20514-1-AP targets BTG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BTG4 fusion protein Ag14339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell translocation gene 4
别名 B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b
计算分子量 223 aa, 26 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC031045
基因名称 BTG4
Gene ID (NCBI) 54766
RRIDAB_10694822
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTG4 antibody 20514-1-APDownload protocol
IHC protocol for BTG4 antibody 20514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols