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验证数据展示

  • WB analysis using 20514-1-AP

    WB analysis using 20514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20514-1-AP (BTG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 40x lens).

BTG4 Polyclonal antibody

BTG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20514-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20514-1-AP targets BTG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BTG4 fusion protein Ag14339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell translocation gene 4
Synonyms B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b
Calculated Molecular Weight 223 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC031045
Gene Symbol BTG4
Gene ID (NCBI) 54766
RRIDAB_10694822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTG4 antibody 20514-1-APDownload protocol
IHC protocol for BTG4 antibody 20514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols