BTK Polyclonal antibody

BTK Polyclonal Antibody for WB, IP, ELISA
Cat No. 21581-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inDaudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy BTK Ready-To-Use IHC Kit. BTK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21581-1-AP targets BTK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 BTK fusion protein Ag16133 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Bruton agammaglobulinemia tyrosine kinase
别名 EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1
计算分子量 659 aa, 76 kDa
观测分子量 70-76 kDa
GenBank蛋白编号BC109079
基因名称 BTK
Gene ID (NCBI) 695
RRIDAB_10734434
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06187
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTK antibody 21581-1-APDownload protocol
IP protocol for BTK antibody 21581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.

Authors - Weijia Sun
humanWB

Int J Mol Med

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Authors - Linlin Sheng
mouseWB

Redox Biol

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway

Authors - Chengli Liu
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
humanWB

Cell Death Discov

Tuning the B-CLL microenvironment: evidence for BAG3 protein- mediated regulation of stromal fibroblasts activity

Authors - Anna Basile
Loading...