验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells |
| Positive IP detected in | mouse spleen tissue |
Planning an IHC experiment? We recommend our IHCeasy BTK Ready-To-Use IHC Kit. BTK primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
21581-1-AP targets BTK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | BTK fusion protein Ag16133 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Bruton agammaglobulinemia tyrosine kinase |
| Synonyms | EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1 |
| Calculated Molecular Weight | 659 aa, 76 kDa |
| Observed Molecular Weight | 70-76 kDa |
| GenBank Accession Number | BC109079 |
| Gene Symbol | BTK |
| Gene ID (NCBI) | 695 |
| RRID | AB_10734434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06187 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BTK antibody 21581-1-AP | Download protocol |
| IP protocol for BTK antibody 21581-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction. | ||
Int J Mol Med Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. | ||
Redox Biol CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway | ||
J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma | ||
Cell Death Discov Tuning the B-CLL microenvironment: evidence for BAG3 protein- mediated regulation of stromal fibroblasts activity |
