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BTLA Polyclonal antibody

BTLA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21300-1-AP

Print datasheet

Synonyms

B and T lymphocyte attenuator, B and T lymphocyte associated, BTLA, BTLA1, CD272

验证数据展示

  • WB analysis of Raji using 21300-1-AP

    WB analysis of Raji using 21300-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21300-1-AP (BTLA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21300-1-AP

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 21300-1-AP

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21300-1-AP targets BTLA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen BTLA fusion protein Ag15884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B and T lymphocyte associated
Synonyms B and T lymphocyte attenuator, B and T lymphocyte associated, BTLA, BTLA1, CD272
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC107091
Gene Symbol BTLA
Gene ID (NCBI) 151888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6A9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
humanIHC

Onco Targets Ther

BTLA Expression in Stage I-III Non-Small-Cell Lung Cancer and Its Correlation with PD-1/PD-L1 and Clinical Outcomes.

Authors - Xiangmin Li
View Article