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验证数据展示

  • WB analysis using 84754-5-RR

    WB analysis using 84754-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84754-5-RR (BTLA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A20 using 84754-5-RR

    IF Staining of A20 using 84754-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using BTLA antibody (84754-5-RR, Clone: 242133E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84754-5-RR

    Affinity and Kinetic Characterization of 84754-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84754-5-RR against Mouse BTLA were performed. The affinity constant is 1.12 nM.

BTLA Recombinant antibody

BTLA Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84754-5-RR

产品说明书

CloneNo. 242133E5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

CD272, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133E5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IF/ICC detected inA20 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84754-5-RR targets BTLA in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 CD272, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133E5
计算分子量34kDa
观测分子量50 kDa
GenBank蛋白编号AAI08964.1
基因名称 Btla
Gene ID (NCBI) 208154
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ32MV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTLA, or B and T lymphocyte attenuator, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

实验方案

Product Specific Protocols
WB protocol for BTLA antibody 84754-5-RRDownload protocol
IF protocol for BTLA antibody 84754-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols