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Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

BTLA/CD272 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241318H5

Cat no : 98096-1-RR

Print datasheet

Synonyms

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

验证数据展示

  • FC experiment of human PBMCs using 98096-1-RR

    FC experiment of human PBMCs using 98096-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98096-1-RR

    FC experiment of human PBMCs using 98096-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98096-1-RR

    Affinity and Kinetic Characterization of 98096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98096-1-RR targets BTLA/CD272 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B and T lymphocyte associated
Synonyms BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator
Calculated Molecular Weight 289 aa, 33 kDa
GenBank Accession NumberBC107091
Gene Symbol BTLA
Gene ID (NCBI) 151888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z6A9
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for BTLA/CD272 antibody 98096-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols