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验证数据展示

  • WB analysis using 68265-1-Ig

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68265-1-Ig

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

BTN2A1 Monoclonal antibody

BTN2A1 Monoclonal Antibody for WB, ELISA
Cat No. 68265-1-Ig

产品说明书

CloneNo. 3A6G2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

BK14H9.1, BT2.1, BTF1, BTN2A1, DJ3E1.1, FLJ36567

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68265-1-Ig targets BTN2A1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 BTN2A1 fusion protein Ag14533 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 butyrophilin, subfamily 2, member A1
别名 BK14H9.1, BT2.1, BTF1, BTN2A1, DJ3E1.1, FLJ36567
计算分子量 60 kDa
观测分子量69 kDa
GenBank蛋白编号BC016661
基因名称 BTN2A1
Gene ID (NCBI) 11120
RRIDAB_2935350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7KYR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)

实验方案

Product Specific Protocols
WB protocol for BTN2A1 antibody 68265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols