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验证数据展示

  • WB analysis using 84550-2-RR

    WB analysis using 84550-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84550-2-RR (BTN2A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84550-2-RR

    Affinity and Kinetic Characterization of 84550-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84550-2-RR against Human BTN2A1 were performed. The affinity constant is 2.73 nM.

BTN2A1 Recombinant antibody

BTN2A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84550-2-RR

产品说明书

CloneNo. 241540H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BTF1, BT2.1, 241540H7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NCCIT cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84550-2-RR targets BTN2A1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 butyrophilin, subfamily 2, member A1
别名 BTF1, BT2.1, 241540H7
计算分子量 60 kDa
观测分子量65 kDa
GenBank蛋白编号BC016661
基因名称 BTN2A1
Gene ID (NCBI) 11120
RRIDAB_3672058
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ7KYR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 65-69 kDa. (PMID: 17785817)

实验方案

Product Specific Protocols
WB protocol for BTN2A1 antibody 84550-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols