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BTNL8 Polyclonal antibody

BTNL8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24959-1-AP

Print datasheet

Synonyms

UNQ702/PRO1347, Butyrophilin-like protein 8, Butyrophilin like protein 8, butyrophilin like 8

验证数据展示

  • WB analysis of MCF-7 using 24959-1-AP

    WB analysis of MCF-7 using 24959-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24959-1-AP (BTNL8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 24959-1-AP

    IHC staining of human stomach cancer using 24959-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24959-1-AP (BTNL8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24959-1-AP targets BTNL8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen BTNL8 fusion protein Ag21203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name butyrophilin-like 8
Synonyms UNQ702/PRO1347, Butyrophilin-like protein 8, Butyrophilin like protein 8, butyrophilin like 8
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC119696
Gene Symbol BTNL8
Gene ID (NCBI) 79908
RRIDAB_2879822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTNL8 antibody 24959-1-APDownload protocol
IHC protocol for BTNL8 antibody 24959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols