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  • Featured Product
  • KD/KO Validated

BUB1 Polyclonal antibody

BUB1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13330-1-AP

Print datasheet

Synonyms

Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A

验证数据展示

  • IF Staining of IMR-90 cells using 13330-1-AP

    IF Staining of IMR-90 cells using 13330-1-AP

    IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY.

  • WB analysis of MKN-45 using 13330-1-AP

    WB analysis of MKN-45 using 13330-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 13330-1-AP

    WB analysis using 13330-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IF/ICC detected inIMR-90 cells, MDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13330-1-AP targets BUB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, canine
Cited Reactivityhuman, carassius auratus
Immunogen BUB1 fusion protein Ag4138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 1 homolog (yeast)
Synonyms Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A
Calculated Molecular Weight 1085 aa, 122 kDa
Observed Molecular Weight 45 kDa, 120 kDa
GenBank Accession NumberBC028201
Gene Symbol BUB1
Gene ID (NCBI) 699
RRIDAB_2814999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43683
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

实验方案

Product Specific Protocols
WB protocol for BUB1 antibody 13330-1-APDownload protocol
IF protocol for BUB1 antibody 13330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanIHC,IF

Cancer Sci

Stathmin guides personalized therapy in oral squamous cell carcinoma.

Authors - Wu-Tong Ju
View Article
Carassius auratusWB

Front Bioeng Biotechnol

A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.

Authors - Wenting Xu
View Article
humanWB

J Exp Clin Cancer Res

Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.

Authors - Liang Chen
View Article
humanWB

Front Pharmacol

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis

Authors - Xie Mengyan
View Article
humanWB,IHC

Thorac Cancer

Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma

Authors - Rui Chen
  • KD Validated
View Article