验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IF/ICC detected in | IMR-90 cells, MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13330-1-AP targets BUB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, carassius auratus |
| 免疫原 | BUB1 fusion protein Ag4138 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | budding uninhibited by benzimidazoles 1 homolog (yeast) |
| 别名 | Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A |
| 计算分子量 | 1085 aa, 122 kDa |
| 观测分子量 | 45 kDa, 120 kDa |
| GenBank蛋白编号 | BC028201 |
| 基因名称 | BUB1 |
| Gene ID (NCBI) | 699 |
| RRID | AB_2814999 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43683 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BUB1 antibody 13330-1-AP | Download protocol |
| IF protocol for BUB1 antibody 13330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas | ||
Front Bioeng Biotechnol A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish. | ||
J Exp Clin Cancer Res Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism. | ||
Front Pharmacol Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis |
