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验证数据展示

  • WB analysis using 28319-1-AP

    WB analysis using 28319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28319-1-AP (BYSL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 28319-1-AP

    IHC staining of human ovary cancer using 28319-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28319-1-AP (BYSL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BYSL antibody included

BYSL Polyclonal antibody

BYSL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28319-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ENP1, Bystin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BYSL Ready-To-Use IHC Kit. BYSL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28319-1-AP targets BYSL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BYSL fusion protein Ag28077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bystin-like
别名 ENP1, Bystin
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC050645
基因名称 BYSL
Gene ID (NCBI) 705
RRIDAB_2881112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13895
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BYSL antibody 28319-1-APDownload protocol
IHC protocol for BYSL antibody 28319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols