验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells |
| Positive IHC detected in | human brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 2 publications below |
产品信息
24412-1-AP targets ATG14/Barkor (C-terminal) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ATG14/Barkor (C-terminal) fusion protein Ag19707 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0831 |
| 别名 | Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L |
| 计算分子量 | 492 aa, 55 kDa |
| 观测分子量 | 65-68 kDa, 42 kDa |
| GenBank蛋白编号 | BC109090 |
| 基因名称 | ATG14 |
| Gene ID (NCBI) | 22863 |
| RRID | AB_2879531 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZNE5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
| IHC protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
| IF protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration. | ||
J Cell Sci Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms. | ||
Microb Pathog Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells. | ||
Exp Eye Res Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis. | ||
EMBO Rep An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration |
