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ATG14/Barkor (C-terminal) Polyclonal antibody
ATG14/Barkor (C-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells |
Positive IHC detected in | human brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 2 publications below |
产品信息
The immunogen of 24412-1-AP is ATG14/Barkor (C-terminal) Fusion Protein expressed in E. coli.
Tested Applications | IF, IHC, WB,ELISA |
Cited Applications | IF, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | ATG14/Barkor (C-terminal) fusion protein Ag19707 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | KIAA0831 |
Synonyms | ATG14, Atg14L, Barkor, KIAA0831 |
Calculated Molecular Weight | 492 aa, 55 kDa |
Observed Molecular Weight | 65-68 kDa, 42 kDa |
GenBank Accession Number | BC109090 |
Gene Symbol | KIAA0831 |
Gene ID (NCBI) | 22863 |
RRID | AB_2879531 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6ZNE5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
IHC protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
IF protocol for ATG14/Barkor (C-terminal) antibody 24412-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Differ ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration. | ||
J Cell Sci Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms. | ||
Microb Pathog Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells. | ||
Exp Eye Res Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis. | ||
EMBO Rep An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration |