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Mouse Bcl2 Monoclonal antibody, PBS Only

Bcl2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66799-1-PBS

Print datasheet

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik

验证数据展示

  • WB analysis using 66799-1-Ig (same clone as 66799-1-PBS)

    WB analysis using 66799-1-Ig (same clone as 66799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 66799-1-Ig (same clone as 66799-1-PBS)

    IF Staining of NIH/3T3 using 66799-1-Ig (same clone as 66799-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (66799-1-Ig, Clone: 1B5B9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

产品信息

66799-1-PBS targets Bcl2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Bcl2 fusion protein Ag24261 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_2882142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.