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验证数据展示

  • WB analysis using 30226-1-AP

    WB analysis using 30226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NCCIT using 30226-1-AP

    IF Staining of NCCIT using 30226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Beta-2-Microglobulin antibody (30226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

B2M, beta 2 microglobulin, HDCMA22P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, U-937 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inNCCIT cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30226-1-AP targets Beta-2-Microglobulin in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, beta 2 microglobulin, HDCMA22P
计算分子量 119 aa, 14 kDa
观测分子量12-14 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_2935535
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
IF protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols