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验证数据展示

  • WB analysis using 30226-1-AP

    WB analysis using 30226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NCCIT using 30226-1-AP

    IF Staining of NCCIT using 30226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Beta-2-Microglobulin antibody (30226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30226-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, U-937 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inNCCIT cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30226-1-AP targets Beta-2-Microglobulin in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, beta 2 microglobulin, HDCMA22P
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight12-14 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_2935535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
IF protocol for Beta-2-Microglobulin antibody 30226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols