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Beta-2-Microglobulin Monoclonal antibody, PBS Only

Beta-2-Microglobulin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C3B7

Cat no : 66207-1-PBS

Print datasheet

Synonyms

B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

验证数据展示

  • WB analysis using 66207-1-Ig (same clone as 66207-1-PBS)

    WB analysis using 66207-1-Ig (same clone as 66207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta-2-Microglobulin antibody included
  • IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta-2-Microglobulin antibody included
  • IF Staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    IF Staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

产品信息

66207-1-PBS targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61769
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.