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CoraLite®650-conjugated Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for
Cat No. CL650-20536

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine, monkey

应用

Actin, ACTB, bate actin, β actin, Beta-actin

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20536-1-AP

产品信息

CL650-20536 targets Beta Actin in applications and shows reactivity with human, mouse, rat, canine, monkey samples.

经测试应用 Application Description
经测试反应性 human, mouse, rat, canine, monkey
免疫原 Beta Actin fusion protein Ag14521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, beta
别名 Actin, ACTB, bate actin, β actin, Beta-actin
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
RRIDAB_2920432
偶联类型 CoraLite®650 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60709
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

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