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CoraLite® Plus 647-conjugated Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey, canine

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL650-20536

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Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin



经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20536-1-AP

推荐稀释比

ApplicationDilution
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of CL650-20536 is Beta Actin Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat, monkey, canine
Immunogen Beta Actin fusion protein Ag14521 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
UniprotP60709
Gene ID (NCBI) 60
RRIDAB_2920432
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Beta Actin antibody CL650-20536Download protocol
Standard Protocols
Click here to view our Standard Protocols