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Conjugate
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Beta TRCP Polyclonal antibody, PBS Only

Beta TRCP Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28393-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

    WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28393-1-AP (Beta TRCP antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BTRC antibody included
  • IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BTRC antibody included
Planning an IHC experiment? We recommend our IHCeasy BTRC Ready-To-Use IHC Kit. BTRC primary antibody included.

产品信息

28393-1-PBS targets Beta TRCP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Beta TRCP fusion protein Ag28739 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-transducin repeat containing
Synonyms
Calculated Molecular Weight 605 aa, 69 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC027994
Gene Symbol Beta TRCP
Gene ID (NCBI) 8945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y297
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTRC, also named as BTRCP, FBW1A, and FBXW1A, is an F-box family protein. It is a Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. BTRC targets many important proteins with diverse functions, such as p53, HRAS, NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation. It is required for the activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB, and TNF. Required for proteolytic processing of GLI3.