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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

    WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28393-1-AP (Beta TRCP antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BTRC antibody included
  • IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BTRC antibody included

Beta TRCP Polyclonal antibody, PBS Only

Beta TRCP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28393-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BTRC Ready-To-Use IHC Kit. BTRC primary antibody included.

产品信息

28393-1-PBS targets Beta TRCP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Beta TRCP fusion protein Ag28739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-transducin repeat containing
别名
计算分子量 605 aa, 69 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC027994
基因名称 Beta TRCP
Gene ID (NCBI) 8945
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y297
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTRC, also named as BTRCP, FBW1A, and FBXW1A, is an F-box family protein. It is a Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. BTRC targets many important proteins with diverse functions, such as p53, HRAS, NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation. It is required for the activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB, and TNF. Required for proteolytic processing of GLI3.