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TBXT Polyclonal antibody

TBXT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20741-1-AP

Print datasheet

Synonyms

Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT

验证数据展示

  • WB analysis of mouse liver using 20741-1-AP

    WB analysis of mouse liver using 20741-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20741-1-AP targets TBXT in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T, brachyury homolog (mouse)
Synonyms Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberNM_003181
Gene Symbol TBXT
Gene ID (NCBI) 6862
RRIDAB_2878730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Brachyury T is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. It binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). The antibody is specific to Brachyury T.

实验方案

Product Specific Protocols
WB protocol for TBXT antibody 20741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols