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验证数据展示

  • WB analysis of mouse liver using 20741-1-AP

    WB analysis of mouse liver using 20741-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TBXT Polyclonal antibody

TBXT Polyclonal Antibody for WB, ELISA
Cat No. 20741-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20741-1-AP targets TBXT in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 T, brachyury homolog (mouse)
别名 Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT
计算分子量 47 kDa
观测分子量 44 kDa
GenBank蛋白编号NM_003181
基因名称 TBXT
Gene ID (NCBI) 6862
RRIDAB_2878730
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15178
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Brachyury T is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. It binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). The antibody is specific to Brachyury T.

实验方案

Product Specific Protocols
WB protocol for TBXT antibody 20741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols