• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Bromobimane

Bromobimane (Monobromobimane) 是一种非荧光物质，但与硫醇反应反应时生成荧光产物。Bromobimane 可被当作一种探针使用，常在临床医学上检验血液中硫化物的含量。

CAS号

71418-44-5

分子式

C10H11BrN2O2

主要靶点

Autophagy

仅限科研使用

Cat No : CM03171

Print datasheet

Synonyms

Monobromobimane

验证数据展示

  • CAS No.: 71418-44-5

    Bromobimane
    CAS#: 71418-44-5

产品信息

Bromobimane is a non-fluorescent substance, but reacts with thiols to produce a fluorescent product.Bromobimane is used as a probe, often in clinical medicine, to test for sulfide levels in blood.

CAS号 71418-44-5
分子式 C10H11BrN2O2
主要靶点 Autophagy
主要通路 自噬
分子量 271.11
纯度 ， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 Monobromobimane

体外活性

Bromobimane in solution (aqueous or organic solvents of medium polarity) react with small thiols, and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimane with thiols are second-order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products.[1] The highly selective, rapid reactivity of bromobimane toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimane an extremely powerful approach to the analysis of low molecular weight biothiols.[2]

溶解度

DMSO:54.22 mg/mL (200 mM)

参考文献

1.Kosower EM, et al. Bromobimane probes for thiols. Methods Enzymol. 1995;251:133-48.
2.Newton GL, et al. Determination of biothiols by bromobimane labeling and high-performance liquid chromatography. Methods Enzymol. 1995;251:148-66.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
=
×
×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2