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EDRF1 Polyclonal antibody

EDRF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21883-1-AP

Print datasheet

Synonyms

C10orf137, RP11 383C5.6, Erythroid differentiation-related factor 1

验证数据展示

  • WB analysis using 21883-1-AP

    WB analysis using 21883-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 21883-1-AP

    IHC staining of human cervical cancer using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 21883-1-AP

    IHC staining of human cervical cancer using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, PC-3 cells, HEK-293 cells, mouse brain tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21883-1-AP targets EDRF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EDRF1 fusion protein Ag16441 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 137
Synonyms C10orf137, RP11 383C5.6, Erythroid differentiation-related factor 1
Calculated Molecular Weight 1238 aa, 139 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC107479
Gene Symbol EDRF1
Gene ID (NCBI) 26098
RRIDAB_11042452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3B7T1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

实验方案

Product Specific Protocols
WB protocol for EDRF1 antibody 21883-1-APDownload protocol
IHC protocol for EDRF1 antibody 21883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia

Authors - Lekha Gandhi
View Article