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C10orf68 Polyclonal antibody

C10orf68 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24975-1-AP

Print datasheet

Synonyms

C10orf68

验证数据展示

  • IHC staining of human kidney using 24975-1-AP

    IHC staining of human kidney using 24975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A549 using 24975-1-AP

    WB analysis of A549 using 24975-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24975-1-AP

    IF Staining of HEK-293 using 24975-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inA549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24975-1-AP is C10orf68 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, monkey
Immunogen C10orf68 fusion protein Ag21682 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 68
Synonyms C10orf68
Calculated Molecular Weight 628 aa, 71 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC125091
Gene Symbol C10orf68
Gene ID (NCBI) 79741
RRIDAB_2879828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

chromosome 10 open reading frame 68 (C10orf68) is a 628 amino-acid protein, which may have three isoforms. The function of C10orf68 is still non-known and needs to be further studied.

实验方案

Product Specific Protocols
WB protocol for C10orf68 antibody 24975-1-APDownload protocol
IHC protocol for C10orf68 antibody 24975-1-APDownload protocol
IF protocol for C10orf68 antibody 24975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols