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验证数据展示

  • WB analysis of MCF-7 using 26212-1-AP

    WB analysis of MCF-7 using 26212-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 26212-1-AP

    IF Staining of MCF-7 using 26212-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using C11orf30 antibody (26212-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

C11orf30 Polyclonal antibody

C11orf30 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26212-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

Protein EMSY, GL002, EMSY, BRCA2-interacting transcriptional repressor EMSY

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26212-1-AP targets C11orf30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 C11orf30 fusion protein Ag23974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 30
别名 Protein EMSY, GL002, EMSY, BRCA2-interacting transcriptional repressor EMSY
观测分子量 140-150 kDa
GenBank蛋白编号BC029375
基因名称 C11orf30
Gene ID (NCBI) 56946
RRIDAB_2880429
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf30 antibody 26212-1-APDownload protocol
IF protocol for C11orf30 antibody 26212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell

EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.

Authors - Antonio Marzio
  • KD Validated
View Article