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C11orf49 Polyclonal antibody

C11orf49 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20195-1-AP

Print datasheet

Synonyms

C11orf49, UPF0705 protein C11orf49

验证数据展示

  • WB analysis of mouse lung using 20195-1-AP

    WB analysis of mouse lung using 20195-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 20195-1-AP

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 20195-1-AP

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20195-1-AP

    IF Staining of HepG2 using 20195-1-AP

    Immunofluorescent analysis of HepG2 cells, using C11orf49 antibody 20195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20195-1-AP is C11orf49 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen C11orf49 fusion protein Ag14123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 49
Synonyms C11orf49, UPF0705 protein C11orf49
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC001860
Gene Symbol C11orf49
Gene ID (NCBI) 79096
RRIDAB_10646431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6J7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf49 antibody 20195-1-APDownload protocol
IHC protocol for C11orf49 antibody 20195-1-APDownload protocol
IF protocol for C11orf49 antibody 20195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Authors - Edward L Huttlin
View Article